Contact Dr. Steven Vogel, NIH and they give cerulean and Venus based linker. They use aminoacid to link the Cerulean and Venus or they may suggest you some alternative.
Steven S. Vogel [[log in to unmask]]

Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http//:www.kcci.virginia.edu
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Workshop on FRET Microscopy, March 9-13, 2010
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christoph Wotzlaw
Sent: Thursday, May 14, 2009 7:41 AM
To: [log in to unmask]
Subject: FRET: ECFP---EYFP construct

Dear colleagues,

for calibration/control experiments I would like to use a ECFP-short linker-EYFP construct (no real protein in-between). Can someone provide me with it kindly? 


Best wishes
Chris

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