If the antibodies available to you are purified and in sufficient 
quantity and concentration (1 - 2 mg/mL), you can directly label the 
antibodies covalently with an appropriate fluorophore.  Fluorophore 
conjugate, or labeling kits are available through Invitrogen:

*http://tinyurl.com/mwabcj

* Invitrogen also has strategies for labeling small quantities of 
antibody non-covalently.  I haven't have very much luck with one such 
kit, called Zenon, but the covalent labeling method has worked well for 
me.  Directly labeled antibodies will certainly work for colacalization 
studies; however, I'm not sure if  they would be appropriate for FRET.

Arne


Ramana Sidhaye wrote:
>
> *Hi-*
>
> *  I am interested in looking at colocalization and/or FRET between 
> two proteins. Unfortunately, the commercially available antibodies for 
> both proteins are not very good, and the antibodies that have been 
> generated by our group and others that do work well are both in 
> rabbit. I was wondering if there was a good protocol or generally 
> accepted mechanism for dealing with this.*
>
> * *
>
> *Thanks,*
>
> *Ramana Sidhaye*
>
> * *
>
>  
>
> Venkataramana Sidhaye, MD
>
> Assistant Professor, Pulmonary and Critical Care Medicine
>
> Johns Hopkins University
>
>  
>
>  
>


-- 

__

Arne K Christensen

Postdoctoral Research Associate
University of Massachusetts, Amherst

USGS Conte Anadromous Fish Research Center
One Migratory Way, PO Box 796
Turners Falls, MA  01376

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