Our lab uses the C1Si which is a quite capable spectral system. (no
company affiliation, just a customer)
Craig
> From your original post it wasn't really clear why you even needed one of
> these high-end machines. There are lower-end systems on the market (Leica
> SPE, Zeiss 700, Nikon C1) which might do all you need and save your company
> quite a bit of money.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
> ________________________________
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Straatman, Dr K.R.
> Sent: Saturday, 25 July 2009 1:46 AM
> To: [log in to unmask]
> Subject: Re: Buying a COnfocal Microscope
>
> Dear Amol,
>
>
>
> We have a SP5 and a FV1000 in our microscope facility. Both are easy to use.
> It is really user dependent which system is preferred. Our Leica system is
> used most, but that has to do with its location in Biochemistry, were we
> have more microscope users. However, the SP5 is also the system with most
> down time because of hardware and software failures. The FV1000 is for sure
> not worse than the SP5. We have the FV1000 now for 2 years without any
> problems. In the past I used an Zeiss510 and was very happy with that system
> as well. I have not worked with the 710, only seen some demos but I would
> imagine that it only has improved. So best is to get the systems in for some
> days and do some testing yourself.
>
>
>
> Good luck
>
>
>
> Kees
>
>
>
>
>
> Dr. Ir. K.R. Straatman
> Senior Experimental Officer
> School of Biological Sciences
> http://www.le.ac.uk/biochem/microscopy/home.html
>
> Postal address:
> Department of Biochemistry
> Henry Wellcome Building
> University of Leicester
> Lancaster Rd.
> Leicester LE1 9HN
> UK
> tel.: + 44 (0)116 229 7085/252 2263
> fax: + 44 (0)116 229 7031
>
>
>
> Times Higher Education University of the Year 2008-9
>
>
>
>
>
>
>
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of regan m
> Sent: 24 July 2009 13:34
> To: [log in to unmask]
> Subject: Re: Buying a COnfocal Microscope
>
>
>
> SP5
>
> THE ONE SUITS U THE BEST ???
>
> On Thu, Jul 23, 2009 at 9:08 PM, Amol K <[log in to unmask]> wrote:
>
> Hello All,
>
> Sorry to bother everyone with these questions but what is a better resource
> than confocal users and specialists! I tried searching for similar topics
> but
> didn't get much useful information.
>
> We will be adding confocal microscope to our facility here at my company.
> I've
> three quotes from Olympus, Leica and Carl Zeiss. The models are Olympus
> FV1000/IX81, Leica TCS SP5 and Carl Zeiss LSM710. Olympus and Leica are
> almost similar in costs whereas Carl Zaeiss is relatively costly for similar
> instrumentation.
>
> My experiment will range from cellular uptake studies of nanoparticles to
> some
> FRET experiments. The nanoparticles will be multi colored and hence spectral
> unmixing will be important. There will be not many users, perhaps 3/4 at
> max.
>
> My questions are how good are these systems when compared to each other,
> which one you will recommend consideing the ease of use, reliability and
> service, any specific advantage of one over another.
>
> I really hope you will take some time out and reply to these questions.
> Thank
> you in advance.
>
> Best,
> Amol
>
>
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