Dear all

Thanks a lot for all the suggestions. We will try and hopefully get nice
results!

:)

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Judy
> Trogadis
> Sent: 30 September 2009 15:26
> To: [log in to unmask]
> Subject: Re: green nuclear stain
> 
> Sytox Green (Molecular Probes) works well for fixed, not sure about live
> cells. Normal dilution is 1:20,000
> but titrate because it is very bright and depending on your setup and
> other dyes may be seen in other channels unless it is at max dilution.
> 
> Judy
> 
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-5046
> [log in to unmask]
> 
> 
> >>> Sylvie Le Guyader <[log in to unmask]> 9/30/2009 5:20 AM >>>
> Hi there!
> 
> 
> 
> Does anyone know of a green nuclear stain that resists fixation? One of
> our
> users wants to do some colocalization of cy3 and cy5 with the nucleus
> and
> our confocal has unfortunately no 405 laser. We are trying to avoid
> NLS-GFP
> but use something that would label all cells right before or after
> fixation.
> 
> 
> 
> Thanks to all!
> 
> 
> 
> Med vänlig hälsning / Best regards
> 
> 
> 
> Sylvie
> 
> 
> 
> @@@@@@@@@@@@@@@@@@@@@@@@
> 
> Sylvie Le Guyader
> 
> Dept of Biosciences and Nutrition
> 
> Karolinska Institutet
> 
> Novum
> 
> 14157 Huddinge
> 
> Sweden
> 
> +46 (0)8 608 9240
> 
>