CONFOCALMICROSCOPY Archives

October 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Peter O'Toole <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 22 Oct 2009 19:31:10 +0100
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Likewise, we have used CyGel. You may need try various strengths to get the 
best lifetime out of the bacteria, especially if they are motile. You will 
also need to seal the edges with vacuum grease to stop it drying out, but 
once in place.

Best

Peter

----- Original Message ----- 
From: "G. Esteban Fernandez" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, October 22, 2009 7:17 PM
Subject: Re: Immobilize bacteria


> I've used CyGel for that purpose before (no commercial interest).
> http://www.biostatus.com/product/cygel/
>
> -Esteban
>
>
> On Thu, Oct 22, 2009 at 1:11 PM, Mike Tighe <[log in to unmask]> 
> wrote:
>> Does anybody have a good way to Immobilize bacteria without killing. We 
>> would like to do a Live/dead stain and our bugs like to float away!
>>
>> Thanks!!
>> Mike
>>
>
> --
> G. Esteban Fernandez, Ph.D.
>
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO  65211
>
> http://www.biotech.missouri.edu/mcc
>
> (573)882-4895
> (573)884-9676 fax
> 

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