Sylvie,

you also might want to have a look at Chromomycin A3.
disadvantage: needs Mg-Ions or will not stain 
(you surely can google some protocols)
advantages: DNA-specific (most others also stain 
RNA); old and patent free and thus comparably cheap (if I remember right).

Steffen

At 15:40 30.09.2009, you wrote:
>Dear all
>
>Thanks a lot for all the suggestions. We will try and hopefully get nice
>results!
>
>:)
>
>Med vänlig hälsning / Best regards
>
>Sylvie
>
>@@@@@@@@@@@@@@@@@@@@@@@@
>Sylvie Le Guyader
>Dept of Biosciences and Nutrition
>Karolinska Institutet
>Novum
>14157 Huddinge
>Sweden
>+46 (0)8 608 9240
>
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[log in to unmask]] On Behalf Of Judy
> > Trogadis
> > Sent: 30 September 2009 15:26
> > To: [log in to unmask]
> > Subject: Re: green nuclear stain
> >
> > Sytox Green (Molecular Probes) works well for fixed, not sure about live
> > cells. Normal dilution is 1:20,000
> > but titrate because it is very bright and depending on your setup and
> > other dyes may be seen in other channels unless it is at max dilution.
> >
> > Judy
> >
> > Judy Trogadis
> > Bio-Imaging Coordinator
> > St. Michael's Hospital, 7Queen
> > 30 Bond St.
> > Toronto, ON M5B 1W8, Canada
> > ph:  416-864-6060  x6337
> > pager: 416-685-9219
> > fax: 416-864-5046
> > [log in to unmask]
> >
> >
> > >>> Sylvie Le Guyader <[log in to unmask]> 9/30/2009 5:20 AM >>>
> > Hi there!
> >
> >
> >
> > Does anyone know of a green nuclear stain that resists fixation? One of
> > our
> > users wants to do some colocalization of cy3 and cy5 with the nucleus
> > and
> > our confocal has unfortunately no 405 laser. We are trying to avoid
> > NLS-GFP
> > but use something that would label all cells right before or after
> > fixation.
> >
> >
> >
> > Thanks to all!
> >
> >
> >
> > Med vänlig hälsning / Best regards
> >
> >
> >
> > Sylvie
> >
> >
> >
> > @@@@@@@@@@@@@@@@@@@@@@@@
> >
> > Sylvie Le Guyader
> >
> > Dept of Biosciences and Nutrition
> >
> > Karolinska Institutet
> >
> > Novum
> >
> > 14157 Huddinge
> >
> > Sweden
> >
> > +46 (0)8 608 9240
> >
> >