>Thanks a lot for your answers.

>Of course I put the slide upside down when using 
>inverted microscope and I asked for Molecular 
>probes about coverslip and they answered me: 
>"The coverslip thickness for the FocalCheck 
>prepared slides is 0.13-0.16 mm" It´s no ideal 
>but I think that this is not the problem.

M. Teresa

SERVICIO de MICROSCOPIA CONFOCAL y CCD
Mª Teresa Seisdedos Domínguez
Oscar Hidalgo Blanco
Amadeo Cazaña Soto
[log in to unmask]
Centro Investigaciones Biológicas (CIB) CSIC
C/Ramiro de Maeztu, 9
28040 Madrid
Phone: + 34-91 8373112 ext.4401
Fax: + 34-91 536 04 32