Dear list,
A colleague is experiencing severe losses of fluorescence in DsRed after
PFA fixation. He is working with Drosophila imaginal discs and would
like to know about other methods of fixation or of good specific
antibodies to DsRed.
Anyone can help?
Thanks,
--
F Javier Díez Guerra, PhD
Profesor Titular
Centro de Biología Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN
phone: +34 91 196 4612
e-mail: [log in to unmask]