Hi Alessandro

We went through the same process last year: choosing a confocal / 2P / FLIM.
We eventually bought the Zeiss LSM710-NLO which we are very happy with so
far although the 2P side has not been extensively used yet. We opted for
FLIM to be placed on an inverted system instead since this suits our
applications better (live cell imaging on glass for FLIM, thick samples
without coverslip for 2P).

The La Vision system was a very serious competitor both for FLIM and the 2P
but as far as I can tell they cannot offer a confocal combined with the 2P.

Hope that helps.

Med vänlig hälsning / Best regards
 
Sylvie
 
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Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
office: +46 (0)8 608 9240
mobile: +46 (0) 73 733 5008

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Alessandro
> Esposito
> Sent: 08 June 2010 23:21
> To: [log in to unmask]
> Subject: non-descanned detectors
> 
> Dear all,
>     we are about to buy a new confocal/two-photon microscope. We are in
the
> middle of an EU tender and this implies I have yet to decide which system
we'll
> purchase.
> 
> I will install detectors for TCSPC on the non-descanned ouput port of the
> future system and I will need to place some optics between the microscope
> and detector.
> 
> Do you have any bad experience (eg, constrains dictated by the design of
the
> microscope) with a particular microscope among the most recent laser
> scanning systems on the market or even in more general terms?
> 
> Kind regards,
> 
> Alessandro
> MRC Cancer Cell Unit, Cambridge
> http://www.quantitative-microscopy.org