Dear list,

I have a question concerning using antibody-conjugated fluorescent beads
for long-time timelapse confocal imaging.

we would like to conjugate fluorescent beads with (rabbit) antibodies,
inject them into zebrafish embryos and track over time. The total number
of images (z*T) will be about 20x50 =1000. If someone has experience with
this kind of experiments, could you tell me:

1. what kind of beads do you use- we would like them to bleach not too fast

and

2. could you share a good protocol for bead-antibody conjugation?

if you have any tips about injecting beads into fish/xenopus embryo, that
will also be great.

Any tips will be very much appreciated!

regards,
Ekaterina



-- 
postdoc Heisenberg lab
Max-Planck-Institute for Molecular Cell Biology and Genetics
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D-01307 Dresden
Germany
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