CONFOCAL MICROSCOPY

4-Day Intensive Hands-On Course

Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm

Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing

7-10 September 2010
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Jo Marrison, University of York
Les Borland, Carl Zeiss
Colin Hornby, Perkin Elmer
and special guest lecture and practical session with Daniel Zicha (CRUK).

Tutorials and practical sessions will cover both basic and advanced 
confocal microscopy. The course will enable participants to realise the 
potential behind many developing confocal techniques and realise the 
simplicity of applying the techniques to a broad range of sample types 
and fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY AIMS
Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on 
laser scanning confocal.
Day 3: Advanced Techniques covering spectral unmixing and FRET with 
fluorescent proteins.
Day 4: Further advances. More samples to explore confocal limits, 
infroduction to MP and time for participants own samples.

Thursday evening will include a special guest speaker and course meal.

The course will utilise many different sample types ranging from 
cultured monolayers to plant cells. A diverse range of fluorescent 
labels will also be used, which will include various fluorescent 
proteins (CFP, GFP and YFP) and classic antibody labels. Over the four 
days, at least four confocal microscopes (2x Zeiss LSM 710 and 2x LSM 
510 META on both an invert and upright microscopes, PerkinElmer 
UltraVIEW VoX) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the 
length of the course.  Evening meals will be provided on both Tuesday 
and Thursday evenings.

Places are limited to 12 participants to permit full hands-on practice, 
so please book early to avoid disappointment.
For further information, please visit the course website at:
www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm

or contact Margaret Newton 01904 328821, [log in to unmask]

-- 
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
PO Box 373
YORK
YO10 5YW
(Sat Nav post code YO10 5DD)

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [log in to unmask]
www.york.ac.uk/biology/tf

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