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August 2010

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Subject:
Time domain FLIM in wide field microscopy
From:
Vanderwinden Jean-Marie <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 25 Aug 2010 03:27:46 -0500
Content-Type:
text/plain
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Dear list members,

I'm seeking opinion from users of PTI TimeMaster™ for time domain FLIM in
wide field microscopy, 
namely its performance, speed of acquisition, bleaching of specimen &
phototoxicity for living cells, software user-friendliness, maintenance
cost, etc…

More generally, how does time domain FLIM in wide field microscopy compare
with FLIM in 2P LSM (besides the confocal optical sectioning feature).

I'm considering the purchase of a FLIM system for our core imaging facility,
which is already equipped with a LSM510NLO & MaiTai laser.
Costs (direct & indirect), user-friendliness and occupancy of the LSM are
among my concerns.

So, wide field or confocal FLIM?, that is my question…

THANKS for your input.

Cheers.

Jean-Marie.

Jean-Marie Vanderwinden, M.D., Ph.D.
Research Director, National Fund for Scientific Research (Belgium)

Laboratoire de Neurophysiologie (Prof. S.N. Schiffmann),
Faculté de Médecine, Campus Erasme, CP 601, 
Université Libre de Bruxelles, 
808 route de Lennik, B-1070 Brussels, Belgium.

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http://www.ulb.ac.be/medecine/neurophy/

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