*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Michael
i suspect the "real" answer might be that there is hardly any way of quantifying fluorescence relative intensity values or at least relating these values to the quantity of fluorochrome at any given subcellular site. I think the scientists prefer such methods as FRET and FLIM because the relevant binding of fluorochrome to something appears more reliable as an indicator of what the experiment is directed at (not grammatical but you know what I mean?).
Carol Heckman
________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Cammer, Michael [[log in to unmask]]
Sent: Thursday, October 28, 2010 9:26 PM
To: [log in to unmask]
Subject: Apotome for intensity measurements?
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Dear microscopists,
I recall from years ago discussions with Zeiss during an one-site demo that while the Apotome produces beautiful images, the deconvolution method of the sequential grid images may not accurately reflect relative intensity values for quantification of fluorescence. Recently I mentioned this to colleagues who have been using the Apotome specifically to look at quantification of chemical species and they got worried about their data. However, there appear to be many peer reviewed published papers where people have used the Apotome for intensity quantification. Does anyone know the real answer?
Sincerely,
Michael
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================
|
