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we did it for proof of principle, fig. 28 in
Feijó & Moreno, 2004, Imaging plant cells by two-photon
excitation,Protoplasma, 223:1-32
(also attached)
basically Paul is on spot (as usual)
Em 07-01-2011 20:17, Paul Herzmark escreveu:
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>
> Hi Michael,
>
> We do it here with the laser tuned to 720. We separate the two Indo peaks
> into two different PMTs by using a 440 dichroic. The emission light going to
> the short wave PMT also goes through a 400/45 bandpass filter. The light to
> the long wave PMT goes through a 480/50 bandpass filter.
>
> Hope that helps!
>
> Paul
>
>
> Paul Herzmark
> Specialist
> [log in to unmask]
>
> Department of Molecular and Cell Biology
> 479 Life Science Addition
> University of California, Berkeley
> Berkeley, CA 94720-3200
> (510) 643-9603
> (510) 643-9500 fax
>
>
> On Fri, Jan 7, 2011 at 9:12 AM, Cammer, Michael
> <[log in to unmask]>wrote:
>
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>>
>> Dear multiphotoners,
>>
>> I'm planning to try using Indo on the multiphoton microscope next week and
>> was wondering whether anybody has tips on best wavelengths to use for
>> excitation (e.g. 720 or 920 nm?) or anything else of potential use.
>>
>> Thank you!
>>
>> Sincerely,
>>
>> Michael
>>
>> ________________________________________________________
>> Michael Cammer, Assistant Research Scientist
>> Skirball Institute of Biomolecular Medicine
>> Lab: (212) 263-3208 Cell: (914) 309-3270
>>
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Jose' A. Feijo', Prof.
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