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I agree w/Michael that the Chroma slides are not the best for MP. I typically use an H&E stained pathology slide. This seems to be stable >3 years
Rich
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York Albany, New York
SS518-474-7048
518-474-4430
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Website www.wadsworth.org/cores/alm/index.htm
Fluorescent plastic slides are not stabile. Depending on their exposure to light they bleach or change color. Using a pulsed laser is a good way to make little burn or other chemical reaction spots in them. Here are two old web pages with some of the problems (and benefits!) explained:
http://www.einstein.yu.edu/aif/instructions/fluor-ref-slides/01.htm
http://www.einstein.yu.edu/aif/instructions/zeiss/liveduo/Zaxis_bleachingtest/index.htm
_______________________________________
Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Mark Cannell [[log in to unmask]]
Sent: Friday, January 14, 2011 7:39 PM
To: [log in to unmask]
Subject: Re: 2P standard
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How about Fluorescent plastic slides -I got some free from Chroma.
Cheers
On 15/01/2011, at 12:34 PM, Boswell, Carl A - (cboswell) wrote:
> Hi all,
> I'm looking for some trustworthy, permanent standard(s) to use in a MP
> system to monitor the condition of detectors and the system in
> general. The goal is a fluorescent source that is immutable. This
> could done either by having unrestricted replacement of bleached label
> with unbleached label (i.e. a solution), or a uniform and very large
> solid, so that it is unlikely that any one tiny volume will be
> imaged repeatedly. We've tried several iterations of an aqueous
> solution of label, but sealing the prep does not prevent eventual
> evaporation. Plus, there seems to be some modification of FITC, for
> example, in solution over time (a month) regardless of exposure to
> light. I'm not sure I can trust the consistency of colored plastic
> slides, since they are not manufactured with this role in mind. One
> possibility is uranyl glass slides, but Corning says they don't make
> these any more. Does anyone know a source? We're also considering
> quantum dots in something like immersion oil , in a sealed chamber but
> I don't have any experience with these labels in a hydrophobic
> environment.
>
> Thanks for your insights.
> C
>
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> Univ. of Arizona
> 520-954-7053
> FAX 520-621-3709
>
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