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I think you're referring to an email I posted about a conversation I had with Iain Johnson at Molecular Probes about the TetraSpeck Beads (no commercial interest). I've copied it here again for you:


***********************
John:

The mounting medium is Saint Gobain Optical Cement.  According to my notes, this has a refractive index of 1.52 when cured.

The spectra for the TetraSpeck green dye are the same as our standard yellow/green FluoSpheres:http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.8811h2o.html

The spectra for the Tetraspeck dark red  dye are the same as our standard dark red FluoSpheres:http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.8807h2o.html

The blue and orange dyes are different from the standard FluoSpheres dyes so I don't have data for those at present.  I'll work on getting it but may take a few weeks.

Best Regards,

iain
***********************

Cheers

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-05-13, at 10:10 AM, Shivaprasad Bhuvanendran wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Hi,
> 
> I do not remember the email exchange but from what I learned at the Optical
> Microscopy course at Woods Hole, Norland Optical Cement # 63 supplied by
> Edmund Scientific should work. The RI for # 63 is 1.56.
> 
> Interestingly, I learned it from another Colin though it was Dr. Colin
> Izzard!
> 
> Best regards,
> Shiva
> 
> PS: No commercial interest.
> 
> Shivaprasad Bhuvanendran
> Research Support Specialist - Bio-Imaging
> The Rockefeller University
> 1230 York Avenue
> Box 209 (DWB 201)
> New York NY 10065
> tel +1 212 327 7487
> fax +1 212 327 7489
> 
> 
> 
> 
> On Fri, May 13, 2011 at 9:43 AM, Colin Rickman <[log in to unmask]> wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Hi
>> 
>> There was an email exchange about fluorescent spheres mounted on slides in
>> an optical cement. I think it was about the prepared Molecular Probes
>> slides. I've tried to search for the email exchange but can't find it. I'm
>> trying to locate the supplier of the optical cement used which had an RI of
>> around 1.5.
>> 
>> Does anybody remember the emails and the supplier?
>> 
>> Thanks
>> 
>> Colin
>> 
>> Dr Colin Rickman
>> Life Science Interface
>> Department of Chemistry (WP 2.03)
>> School of Engineering and Physical Sciences
>> Heriot-Watt University
>> Edinburgh
>> EH14 4AS
>> 
>> Tel: +44 131 4514193 (Office)
>> 
>> http://www.lifescienceinterface.hw.ac.uk
>> http://www.eps.hw.ac.uk/departments/chemistry/cr.htm
>> 
>> 
>> 
>> --
>> Heriot-Watt University is a Scottish charity
>> registered under charity number SC000278.
>>