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1 mm agar plus a 1mm+ plastic base are not ideal imaging conditions. A  
long working distance objective will focus but the thick plastic base  
will degrade the image - most objectives are designed for 0.17mm  
glass. But how good do the images need to be for your purposes ?

A solution is glass bottomed multiwell plates, but they are relatively  
expensive. Given the popularity of multiwell plates for imaging there  
is probably a decent market for a plate with a suitably thin plastic  
bottom.




Quoting "Claire Brown, Dr." <[log in to unmask]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>
> We have a lab that wants to screen a C. Elegans genetic library with  
> about 18,000 genes. They want to take images of the worms on 24 well  
> plates and measure the distance between 2 of 3 EGFP spots on the  
> worms.
>
> We would like to do the experiments on an inverted HCS automated  
> microscope. The problem is they have the worms growing on a ~1 mm  
> thick nutrient-rich agar layer.
>
> We need to image the worms through the agar so we need to find a  
> different way to prepare the samples. Has anyone ever tried  
> something like this? If so do you have a solution for this? The agar  
> is poured by hand so getting it much thinner than 1 mm would be  
> difficult.
>
>
> Sincerely,
>
> Claire
>
> --------------------------------------------------------------------------------
> Claire M. Brown, PhD
> McGill University
> Life Sciences Complex Imaging Facility Director
>



Jeremy Adler
IGP
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