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October 2011

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From:
Patrick Van Oostveldt <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 10 Oct 2011 00:03:39 +0200
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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear

Here some references at least for microbes. For furtehr details look  
at the website of our colleague Willy Verstraete.

If yoy like specific antifungal effects, this is more complex. But  
what is routinely used is treatment of the containers with ultrasound,  
which inhibits also development of algae.

References:

Sintubin, L., B. De Gusseme, et al. (2011). "The antibacterial  
activity of biogenic silver and its mode of action." Applied  
Microbiology and Biotechnology 91(1): 153-162.

Chopra, I (2007). "The increasing use of silver-based products as  
antimicrobial agents: a useful development or a cause for concern?".  
The Journal of antimicrobial chemotherapy 59 (4): 587?90.

Greetings,

Patrick Van Oostveldt
Quoting "Mark Cannell" <[log in to unmask]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Do you have a reference for the antifungal activity of simple  
> metallic silver? I've heard this repeated many times but never found  
> a reputable source.
>
> Cheers
>
>
> On 7/10/2011, at 10:26 PM, Patrick Van Oostveldt wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Including some part of the tubing by a copperpipe can solve a lot.
>> If more money is available a small silver pipe can also be used.
>>
>> Patrick
>>
>> Quoting "Dan Focht" <[log in to unmask]>:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Ekatrina
>>>
>>>
>>> I am not sure about ThermoTek brand chillers but we use a 10%  
>>> alcohol and water in our recirculating chiller and have never had  
>>> anything grow in it.
>>>
>>>
>>> Dan
>>>
>>>
>>>
>>>
>>>
>>> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear all,
>>>
>>> Need your advice regarding the contamination of our MP cooling.
>>>
>>> We were filling our ThermoTek chillers (T255P) that are connected  
>>> with Cameleon II MP lasers with  distilled water, according to  
>>> Coherent instructions.
>>> Since few days, we got dramatic contamination (some algae or  
>>> bacteria growing) in the water.
>>>
>>> Even if we flash a chiller with 10 l water and refill new, we get  
>>> already some visible contamination next day. One of the systems  
>>> was clogged once.
>>>
>>> Are there any tips of how to get the growth out?
>>> We thought of adding 5% ethanol instead of water. Or building  
>>> bacterial filters (like on FACS machines) in the flow circle.
>>>
>>> Does someone have experience with these two approaches, and is  
>>> there something better?
>>>
>>> Looking forward to many helpful tips! Nice weekend to everyone,  
>>> except those who have to flush their chillers :)
>>>
>>> Ekaterina
>>>
>>> ___
>>>
>>> Ekaterina Papusheva,
>>> Imaging Facility Manager
>>>
>>> Institute of Science and Technology Austria
>>>
>>> Phone  +43-(0)2243 9000-1043;
>>> Mobile +43-(0)664 88509130
>>> Fax    +43-(0)2243 9000-2000
>>>
>>> Am Campus 1
>>>
>>> A-3400 Klosterneuburg
>>>
>>> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>>>
>>>
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>>>
>>> Dan Focht
>>> Bioptechs, Inc.
>>> 3560 Beck Rd.
>>> Butler, PA 16002
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>>> P: (724)282-7145
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>>> [log in to unmask]
>>>
>>
>>
>>
>> --
>> Dep. Moleculaire Biotechnologie
>> Coupure links 653
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>>
>> tel 09 264 5969
>> fax 09 264 6219
>



-- 
Dep. Moleculaire Biotechnologie
Coupure links 653
B 9000 GENT

tel 09 264 5969
fax 09 264 6219

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