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Out of interest, is the limitation that the water layer is only 20-30  
microns thick or that the objective really needs to be that close ?

a 20-30 micron thick layer of water can easily by sandwiched between a  
slide and a coverslip using microspheres as spaces. Then image with a  
water objective that works thru a coverslip.




Quoting "MODEL, MICHAEL" <[log in to unmask]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> 20-30 microns in water. I doubt such lenses exist but who knows... Thanks!
>
> Mike
>
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] On  
> Behalf Of Martin Wessendorf [[log in to unmask]]
> Sent: Thursday, December 08, 2011 9:20 PM
> To: [log in to unmask]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> Dear Mike--
>
> How close do you need to get and what kind of resolution do you need?
> And in what medium?  Air?  Water?  Oil?
>
> Good luck!
>
> Martin
>
> On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:
>
>> Hi Martin - that's correct, I want to bring the objective very  
>> close to the specimen, almost touching it.
>>
>> -----Original Message-----
>> From: Confocal Microscopy List  
>> [mailto:[log in to unmask]] On Behalf Of Martin  
>> Wessendorf
>> Sent: Thursday, December 08, 2011 4:27 PM
>> To: [log in to unmask]
>> Subject: Re: small and variable working distance
>> Dear Mike--
>>
>> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>>
>>> Dear Confocal Microscopists - Does anyone of an objective with  
>>> variable, down to zero, working distance? Thanks!
>>
>> I'm not sure I'm getting the concept--do you want an objective that
>> *needs* to be in contact with the specimen to be in focus?  --It might
>> help me if you explained a bit more about what you're trying to do.
>>
>> Thanks--
>>
>> Martin Wessendorf
>
> --
> Martin Wessendorf, Ph.D.                   office: (612) 626-0145
> Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
> University of Minnesota             Preferred FAX: (612) 624-8118
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