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We have a Zeiss 710 with three spectral detectors. Detection is fine up to 740 nm. Between 740 and 750 nm the instensity falls off quickly. Nothing is detectable above 750 nm. We measured this by reflection of a MaiTai laser.
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Judy Trogadis [[log in to unmask]]
Sent: Friday, December 09, 2011 8:34 AM
To: [log in to unmask]
Subject: Re: Near IR Imaging
We are trying to image ICG dye (indocyanine green, Ex. ~ 800nm) and getting a filter cube was simple. The difficulty is finding a light source that emits at a high enough frequency and making sure the detector, in our case, a CCD camera can see it. Manufacturers often put IR filters in both the camera and light source against potential heat damage so those have to be removed.
I also found information about light source output from companies to be inconsistent as if they don't have too much experience with this range of wavelengths. Currently, we are dusting off an olde Xenon light source and will take a look.
Judy
Judy Trogadis
Bio-Imaging Coordinator
Keenan Research Centre, St. Michael's
209 Victoria Street
Toronto M5B 1T8, Canada
office: 416-864-6060 ext. 77612
imaging facility: ext. 77434
cell: 416-909-9878
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Kim Peifley
Sent: Thursday, December 08, 2011 4:06 PM
To: [log in to unmask]
Subject: Near IR Imaging
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Does anyone have suggestions on how to set up a microscope for imaging
Near IR dyes? We would like to image Cy7, Cy7.5 and VisTag S750. We have
Zeiss 510, 710 microscopes as well as Olympus FV1000 and TIRF3 microscopes.
Thanks for your help.
Kim Peifley
Kim Peifley (Contractor)
Research Associate I
Optial Microscopy and Analysis Laboratory
http://atp.ncifcrf.gov
SAIC-Frederick, Inc.
National Cancer Institute at Frederick
Post Office Box B
Frederick, MD 21702
Phone: 301-846-6561
Fax: 301-846-7672
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