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Hi  List,

We are trying to image uptake of a Pyrene based dye by some cells using
two-photon excitation (as we don't have a UV confocal system). The
pyrene is excitable at 345nm and emits at 380nm. We did some trials with
straight dye and found we can excite it quite well in the 740-780nm
range. Turns out that is where mitochondria light up too due to the
large amounts of NADH they have. Very nice staining, who needs
mitotracker. 

Does anyone have any secrets on how to get around this?

Cheers

Cam


Cameron J. Nowell
Microscopy Manager 
Centre for Advanced Microscopy 
Ludwig Institute for Cancer Research Melbourne - Parkville Branch 
PO Box 2008 
Royal Melbourne Hospital 
Victoria, 3050 
AUSTRALIA 
Office: +61 3 9341 3158 
Mobile: +61 422882700 
Fax: +61 3 9341 3104 
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