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January 2012

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Confocal Microscopy List <[log in to unmask]>
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Mon, 16 Jan 2012 01:08:20 +0000
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There isn't any difference between widefield and confocal resolution in any normal fluorescence mode.   Do you really need an app to work out 0.61 lambda/NA?  And then to divide by 2.3 to get Nyquist?  If you are in multiphoton it's a little more complicated since root 2 (1.414) comes in, but you can just use the formula 0.43 lambda / NA.  

					Guy

Optical Imaging Techniques in Cell Biology
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Tim Feinstein
Sent: Monday, 16 January 2012 4:54 AM
To: [log in to unmask]
Subject: Re: Light Lab by Carl Zeiss Microscopy for iOS devices

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi John, 

ResCalc is a nice app, but it seems to calculate res and nyquist for widefield microscopy only.  Am I wrong about that?  As it is I use the Nyquist calculator on the SVI site (no commercial interest) but I would love to have something off-line.  

Cheers, 


TIm Feinstein

Sent from my iPad

On Jan 15, 2012, at 10:40 AM, John Oreopoulos <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Adrian, there is a fairly good iPhone application that does some of the things you mentioned. It's called ResCalc.
> 
> John Oreopoulos
> Research Assistant
> Spectral Applied Research
> Richmond Hill, Ontario
> Canada
> www.spectral.ca
> 
> On 2012-01-15, at 1:03 AM, Adrian Smith <[log in to unmask]> wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> On 14/01/2012, at 9:01 PM, Johannes Amon wrote:
>> 
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>> 
>>> of course I can't tell you any specifics but at the moment we are evaluating a native port to 
>>> android ICS 4.0. at the end it always comes down to budget so it would help immensely if 
>>> you'd order some confocals right now ^^
>>> 
>>> just joking, gonna keep you posted on this project
>> 
>> 
>> I would love to find a decent resolution calculator that can be used on a smartphone/tablet (iOS preferably for me :)
>> 
>> Add in various options for different digitisation approaches (PMT, cameras) with Nyquist shortcuts and it would be real winner…
>> 
>> Regards,
>> 
>> Adrian Smith
>> Centenary Institute, Sydney, Australia

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