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Hi list!
One quick question on the side of the LED topic: what is your favourite device to measure the excitation light power at the object plane?
I saw that Xcite has one (http://www.ldgi-xcite.com/products-xr2100-xp750.php) as well as Gigahertz (http://www.gigahertz-optik.de/357-0-PT-9610+mit+PD-2T.html).
Does anyone have some experience with them or others?
Med vänlig hälsning / Best regards
Sylvie
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Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14183 Huddinge
Sweden
office: +46 (0) 8 5248 1107
LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Justin Ross
> Sent: 27 February 2012 08:21
> To: [log in to unmask]
> Subject: Re: LEDs
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
> My experience has been similar to Carl's, I have almost never gotten an
> actual emission spectra for a solid state light source. Hence I always
> measured it myself. The manufacturer just quotes a centre wavelength and
> sometimes a bandwidth. Maybe I'm overly cautious but I do the same for
> optical filters too, especially older coloured glass ones. IMO it is
> very important to know the actual spectrum of the light source.
>
> Regards,
> Justin.
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Boswell, Carl A - (cboswell)
> Sent: Saturday, 25 February 2012 3:12 AM
> To: [log in to unmask]
> Subject: Re: LEDs
>
> Hi Guy,
>
> My reference to lack of spectra came from the online documentation for
> API's InsightSSI
> http://www.api.com/downloads/pdfs/lifescience/InsightSSI.pdf (no
> spectra) and X-Cite's XLED1's
> http://www.ldgi-xcite.com/downloads/brochures/LDGI-XCite-XLED1-FAQ-2012.
> pdf separate LED spectra. Lumencor's SOLA light engine brochure
> http://www.lumencor.com/products/sola_product_sheet.html is the closest
> I've seen to a useable spectrum.
>
> In fact, thanks to Lumencor's openness the SOLA spectrum is on the
> database, www.spectra.arizona.edu. With the ability to juxtapose (and
> download) this spectrum with spectra from the listed filters, mirrors
> and labels, the value to the imaging community of making these light
> source spectra available becomes more obvious.
>
> Cheers,
> C
>
>
> Carl A. Boswell
> 520-742-6131
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Guy Cox
> Sent: Friday, February 24, 2012 6:36 AM
> To: [log in to unmask]
> Subject: Re: LEDs
>
> *****
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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Well, I think they do. The second edition of my book 'Optical Imaging
> Techniques in Cell Biology' has spectra from one of the major players in
> the field. I do think that this data is freely available. Has anyone
> actually had trouble getting these spectra?
>
>
> Guy
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Boswell, Carl A - (cboswell)
> Sent: Friday, 24 February 2012 1:49 PM
> To: [log in to unmask]
> Subject: Re: LEDs
>
> "If you have a spectrometer available I suggest scanning the emission to
> get the real emission spectrum of the LED."
>
> It would be of more general use if the manufacturer supplied a
> standardized, trustworthy spectral output of these devices. Then
> everyone could determine for themselves what would work best for their
> systems and samples without the need for additional instrumentation.
> C
>
>
> Carl A. Boswell
> 520-742-6131
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Justin Ross
> Sent: Thursday, February 23, 2012 6:14 PM
> To: [log in to unmask]
> Subject: Re: LEDs
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> Hi,
> We previously had the Xe lamps and changed to the SSI illumination on
> our Deltavision Core. The exposure times were reduced by a factor of 5
> to 10. The SSI is actually made by Lumencor (no commercial interest).
> From my experience it works really well without the downsides of a lamp
> of heat generation or having to align and change bulbs.
> If you do decide to use LEDs, I strongly suggest still using bandpass
> excitation filter (as suggested previously) because with some LEDs there
> upto 5% light emission at wavelengths upto 50 nm higher than the peak
> which will generally add a lot of background to your images. If you have
> a spectrometer available I suggest scanning the emission to get the real
> emission spectrum of the LED.
>
>
> Regards,
> Justin.
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Julio Vazquez
> Sent: Friday, 24 February 2012 4:14 AM
> To: [log in to unmask]
> Subject: Re: LEDs
>
> *****
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>
> Hello all,
>
> Applied Precision just told me that the InsightSSI illuminator is
> actually not LED based, so please ignore my previous posting, as far as
> LEDs are concerned.
>
> Julio.
>
> ==
>
>
> On Feb 23, 2012, at 2:06 AM, jacques wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Thanks for your help and comments !
> >
> > Jacques
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