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February 2012

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Subject:
How "deep" can you see in a brain slice?
From:
Petr Busek <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 5 Feb 2012 13:53:33 -0600
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Dear all, 
I am trying to view fluorescently labeled glioma cells invading into a 400um
thick brain slice on an Olypus FV300. Has anyone experience with this and
how "deep" it is reasonable to expect to see in the slice using a confocal
microscope? How can you maximize this depth? (selection of objectives,
processing of the slice....)
Thanks for any suggestions, Petr. 

Petr Busek, MD, PhD
Charles University in Prague 
First Faculty of Medicine
Laboratory of Cancer Cell Biology
Institute of Biochemistry and Experimental Oncology
U Nemocnice 5
128 53 Prague 2
Czech Republic
www.lf1.cuni.cz/lbnb
 Fax +420 224 965 826

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