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I guess the sample is not only dead (in vacuum?) but also destroyed inthe imaging process. The only time we used a focussed ion beam was to drill small holes in metal with better than optical precision. A cool technique, but for my taste the paper could have some more information about how the sample is prepared and comparisson to other high resolution techhniques. What is an imersion objective in the cotext of ion beam microscopy?
best wishes
Andreas
-----Original Message-----
From: Steffen Dietzel <[log in to unmask]>
To: CONFOCALMICROSCOPY <[log in to unmask]>
Sent: Thu, 9 Feb 2012 12:46
Subject: Re: 30x30x1 nm, 7 colors
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This MIMS seems like a cool technique, if your question can be solved by
investigating the surface of a dead sample (at least I assume the sample
is dead, if not before then after the ion bombardement; certainly if the
cell surface was sputtered as for Steinhauser et al. Fig 1a).
They say the maximum field size is 80 x 80 µm (Methods to Steinhauser et
al.) With an xy-resolution of 28 nm (Supp Fig 1 legend) according to the
Nyquist criterion that would be >6500 px squared, probably too many. But
let us assume a standard 512x512 px frame for some appropriate ROI, has
anybody an idea what the frame time is?
Steffen
On 07.02.2012 04:14, George McNamara wrote:
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>
> 30x30x1 nm, 7 colors ... see figure 1 for colors - the 7 'colors' can
> probably be extended to more for a bit more money.
> http://www.nature.com/nature/journal/v481/n7382/extref/nature10734-s1.pdf
> The same lab has the adjacent article -
> http://www.nature.com/nature/journal/v481/n7382/extref/nature10745-s1.pdf
>
> 30x30x1 nm (yes, nanometer) is very nice - perhaps the fluorescence
> nanoscope developers should spend a bit less time on pushing from the
> current ~20x20x50 nm (which should get to 10x10x25 nm by a certain
> improvement in light output while on) and spend a bit more time on
> producing making new biomedical discoveries.
>
> I may say a few words about this approach during my short talk at the
> ABRF nanoscopy session in Orlando in late March.
> I might start the talk with the nice "SnapShot; light Microscopy" in
> Cell (Nov 23, 2011 issue - I was able to download from home)
>
> http://download.cell.com/pdf/PIIS0092867411013535.pdf?intermediate=true
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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Marchioninistr. 15, D-81377 München
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