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Dear all,
we are trying to image fixed cell spheroids (>100um diameter) on a leica 
sp5 inverted confocal microscope. The high resolution objectives we use 
have short but sufficient working distance to analyse the spheres if 
they attached to the  coverslip.  However standard coatings as 
polilysine are not efficient in immobilizing them (even if they can 
initially adhere we loose them extensively during staining). We tried  
also particular gels as Q gel but we were not able to find a 
reproducible protocol to have them clearly attached to the substrate  
instead of being simply embedded in the gel.
I know that new high NA water-immersion long working-distance objectives 
could provide the simplest solution but unfortunately their cost is 
quite limiting.
thanks for all the answers
Mario

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---PLEASE Note the change in telephone number---

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Mario Faretta
Department of Experimental Oncology
European Institute of Oncology
c/o IFOM-IEO Campus for Oncogenomics
via Adamello 16 20139 Milan Italy
Phone: ++39-0294375027
email: [log in to unmask]
http://www.ifom-ieo-campus.it