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March 2012

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From:
"Vergara, Leoncio A." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 13 Mar 2012 03:07:28 +0000
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I second the opinion of Cameron, his description is almost exactly our experience. I find the automation tools in metamorph a very strong point, I almost don't do any analysis with NIS, however I find NIS better than metamorph for examining multidimensional datasets, it is very intuitive. We also use metamorph (and NIS) in combination with Image J and Imaris, I don't think there is a single package that does everything. 

Leoncio Vergara
Assistant Professor
Center for Biomedical Engineering
University of Texas Medical Branch
Galveston, Texas  

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cameron Nowell
Sent: Monday, March 12, 2012 8:56 PM
To: [log in to unmask]
Subject: Re: MetaMorph versus Elements?

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Hi Michelle

Do you want to use them for anlaysis of driving hardware?

We have both but use them for different things. NIS AR is used primarily for image capture on a couple of our systems. The EDF (Extended depth of
focus) is really useful for some of our imaging. We do not use NIS for image processing (other than EDF) or analysis. NIS has all the usual tools for image processing and analysis but the automation and scripting can be a bit tricky to get your head around. We use MetaMorph for capture as well (automated tile scanning and live cell imaging).

For all our analysis we use a combination of MetaMorph, Fiji and Imaris.
The bulk of the work is usually done in MetaMorph because its ease of scripting/automation. Part of that ease comes from what you are familiar with of course, but I find MetaMorph's journal editor to be easier to use (with better available tutorials etc) than NIS.


Cost could be an issue as MetaMorph is going to be double the price of NIS (for analysis). A metamorph capture system will be more than double NIS.

If you are interested in some tutorials etc for MetaMorph I am happy to send you a link to download a heap of training notes (330 page manual) and demo images I have put together over the last two years for a MetaMorph training course I co-ran here in Melbourne.

The usual statements apply - no commercial interests in any of the platforms just a happy (sometimes frustrated) user.

Cheers

Cam


Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA
Office: +61 3 9341 3158
Mobile: +61 422882700
Fax: +61 3 9341 3104
Facility Website
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Michelle Aloni
Sent: Tuesday, 13 March 2012 11:29 AM
To: [log in to unmask]
Subject: MetaMorph versus Elements?

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Dear all,

If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. 

If you used both of them, how do they compare? Which one would be your personal choice and why?

Thank you ahead of time

Michelle Aloni MS, HTL (ASCP)
Research Specialist
USC Keck School of Medicine

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