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April 2012

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Subject:
From:
Sylvie LeGuyader <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 4 Apr 2012 15:46:53 +0000
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Hi Lai

Yes we have had that luck too...
Ask Andor to update (with a CD, not just downloading the file) the Olympus software which runs in the background of iQ. That solved it for us. Pity it took one year...

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14183 Huddinge
Sweden
office: +46 (0) 8 5248 1107 
LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008


> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Lai Ding
> Sent: 04 April 2012 17:35
> To: [log in to unmask]
> Subject: Problem with Olympus ZDC1
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear Listers,
> 
>   We have an ongoing Olympus ZDC1 problem which has troubled us for nearly a
> month. I hope the list can help us identify the source of the problem and lead to a
> solution.
> 
>  We have an Andor Revolution spinning disk (Yokogawa) system connected to an
> Olympus IX81 inverted microscope stand with ZDC1 installed.  The system works
> fine until about 4 week ago, the ZDC failed during a routine time-lapse scan and
> cause error indicator flashing on the Olympus UCB controller. We have to switch
> off the UCB control box to erase the error flag.  Since then the ZDC kept failing
> under various conditions (listed below),
> 
> A.    We tested on oil and water objectives.
> B.    We tested on different chamber temperature:  37C and room T.
> C.   We tried different ZDC settings.
> D.   It seems the failure of ZDC happened at random time point. Sometime it
> failed after 5 minutes of the start of a time-lapse scan; sometime it runs OK for an
> overnight scan then failed in the morning.
> E.     We suspect it is caused by electronic interference with other equipment,
> so we grounded the UCB control box. We changed the cable coupling to put UCB
> on its own dedicated squid, but it didn't solve the problem.
> 
>   I wonder if any group with ZDC1 had experienced similar problem before, any
> suggestion are welcome.
> 
>  Best
> 
> Lai

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