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Hi, Dave,
We have went through same so called "acquisition automatic stop" problem on our wide field fluorescence microscope, Olympus BX61 WI, with the photometrics Coolsnap ez camera and MediaCybernetics software IPP. After about two years testing (tried almost the same things as what you have done), we finally changed the camera to Olympus xm10 and Olympus Cellsens Dimension. In my case, I suspect that the problem came from the mirror unit but the 3rd part software was not able to point out the error so the acquisition was stopped without any error message most of the time.
If I were you, I would like to ask Olympus try their acquisition software and camera on your system to find out if it will solve your problems the next.
The best,
TONG Yan (Ms) :: NUS Centre for BioImaging Sciences :: Department of Biological Sciences :: National University of Singapore :: 14 Science Drive 4, Singapore 117543 :: (65) 6516 7202 (DID) :: (65) 6779 2486 (Fax) ::) :: [log in to unmask] (E) :: www.nus.edu.sg (W)Company Registration No: 200604346E
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From: Confocal Microscopy List [[log in to unmask]] On Behalf Of David Johnston [[log in to unmask]]
Sent: Thursday, April 05, 2012 3:18 PM
To: [log in to unmask]
Subject: Problem with Olympus IX81 system
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Dear All,
not directly confocal related but I guess that other folks will be in facilities
with other high end microscopes as well.
We have a fully automated Olympus IX81 time lapse system with prior stage
and SIS camera running under Cell^P. It behaves beautifully on phase contrast
only and fluorescence only runs, but when we do combined phase and
fluorescence (MFIP mode) runs, we experience random system crashes at
different times during the run on about 50% of occasions. This problem has
been ongoing for about 4 years now and neither Olympus nor I have come up
with the cause. We have had a loan system and swapped out every
automated part in turn, rebuilt the entire software installation (including off-
network operating system) several times, tried logging serial port coms etc
etc, all to no avail. We still can't even determine if it a software or hardware
issue.
Does anyone have a similar system and if so, have they experienced similar
issues / are they able to run combined phase / fluorescence runs without a
problem?
Thanks in advance for any suggestions, feedback and ideas as Olympus and I
have run out of ideas!!
Dave Johnston,
Biomedical Imaging Unit,
University of Southampton.
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