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April 2012

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Subject:
Mica mounting for TIRF SPT
From:
Artur M <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 9 Apr 2012 01:51:01 -0500
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Dear all, 

do any of you have an experience with mounting mica on coverslips for TIRF-SPT 
(SPT of labeled lipids in supported lipid membranes)? So far I was doing it by 
gluing thin slices of mica to coverslips with NOA63, but I think thickness is still to 
high and I'm loosing a lot of resolution (and signal). It will be fine for 'normal' 
fluorescence studies, but I can forget about SPT. Any hints? 
Thanks, 
AM.

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