CONFOCALMICROSCOPY Archives

April 2012

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Subject:
Re: Optimal two-photon dual-color imaging using eGFP and . . . .?
From:
Andrew York <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 13 Apr 2012 11:39:55 -0400
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*****
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No personal experience, but these links seem relevant:

dx.doi.org/10.1038/nmeth0508-373
dx.doi.org/10.1038/nmeth.1596

On Fri, Apr 13, 2012 at 11:21 AM, Eric Olson <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List,
>
> We are imaging cortical neurons in a transgenic mouse embryo (eGFP
> expressing). We can express a second FP by electroporation and want to
> visualize both FPs at one excitation wavelength.
>
> Any recommendations for another FP partner for eGFP? Possibly DsRed?  And
> what excitation wavelength is used to excite both FPs? Our Chameleon laser
> tunes 700 to 950nm, but without much energy at the longer wavelengths.
>
> Thanks,
>
> Eric
>
>
> Eric C. Olson, Ph.D.
> Department of Neuroscience and Physiology
> SUNY Upstate Medical University
> 3295 Weiskotten Hall
> 766 Irving St.
> Syracuse, NY 13210
>

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