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April 2012

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Subject:
FLIM in fixed samples
From:
Javier Adur <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 16 Apr 2012 11:31:43 -0300
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Dear all. 

I am interesting in to know if is valid get FLIM
images from unstained but fixed tissues. Is possible evaluate some biochemical
(FAD or NADH ) information from these images? I already find some works using
FLIM in fixed samples (Conklin et al., 2009 Cell Biochem Biophys DOI
10.1007/s12013-009-9046-7; Tadrous et al., 2003 Journal of Pathology DOI
10.1002/path. 1286, etc.). But I have also read that does not make sense to
work with FLIM in this kind of samples. 

 I appreciate any imput about this question. 

Thanks in advance. 

Javier.





                     
                    Javier F. Adur, Ph.D.
Optics and Photonics Research Center (CEPOF)
Biophotonic Group - IFGW - UNICAMP - BRASIL   
  
              

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