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Hi Carlos,

Check whether or not you have the image being scaled.

You can look at "Edit - Options - Conversion Options". Turn off "Scale when Converting".

Also, if you are using Bioformats to open the files, then make sure "Autoscale" is turned off in the Bioformats Import Options under Color.

Hopefully, this will help.

If it still looks different, it might be that your monitor is very different from the one on the scope.

Kind regards,

Jacqui

Jacqueline Ross 

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences 
The University of Auckland 
Private Bag 92019 
Auckland, NEW ZEALAND 

Tel: 64 9 373 7599 Ext 87438 
Fax: 64 9 373 7484 

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Carlos Lizama
Sent: Tuesday, 29 May 2012 7:48 a.m.
To: [log in to unmask]
Subject: question about imagej

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hi,

Im working with imagej. but i have a problem. All the time when I acquired the 
photos from library (.lif) the leica confocal scope, the quality of the image is 
completly different the original observed in the leica scope. Somebody know 
How Can I set up imageJ to get the same quality?   


thanks carlos