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Hi,

At a certain point he had our 510 with SHG capability. In practice you need to direct couple the laser (one inch northwest from the visible laser fiber you should have a hole), change some dichroic mirrors and ask a quote for a PMT on the forward detection. Alignment is pretty easy by using a visible laser reflection. There might be further details though, namely the ms access db configuration.

Do not expect a good performance...we had better signal with http://www.sciencedirect.com/science/article/pii/S0968432804000897  than with the zeiss, both forward and backward direction.

Have a look at the objectives specs. Don't forget that you might be imaging at 800 and collecting at 400.

All the best,
NM

PS: Don't forget
http://www.facebook.com/CoreManagementWorkshop



On May 3, 2012, at 3:30 PM, Owens, Peter wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear all,
> 
> I would like to use a ziess axiovert  microscope (LSM 510 confocal unit)
> coupled to a spectra physics tsunami multiphoton laser to image the shg
> signal from collagen fibres in heart valves.  
> 
> I am wondering what is the best way to do this and can anyone give
> comments / recommendations on how our  existing machine may be
> retrofitted for this task.
> 
> Thanks
> 
> Peter Owens 
> 
> 
> __________________________________
> Dr. Peter Owens
> Centre for Microscopy and Imaging and NBIPI
> National University of Ireland Galway
> Landline: 0035391494036
> Mobile:    00353863326749
> ------------------------------------------------------
> 
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Mark Cannell
> Sent: 02 May 2012 17:21
> To: [log in to unmask]
> Subject: Re: confocal PMT intermitently dies
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Check connectors...  Unplug and replug all connectors. If that doesn't
> work then its most likely electronic in the pmt3 power supply or pmt3
> preamp stages...
> 
> Hope this helps
> Mark
> 
> On 2/05/2012, at 4:22 PM, Arvydas Matiukas wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Dear list,
>> 
>> 
>> Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs of 
>> powering on, all image pixels turn to  zero intensity no matter what 
>> are laser power, pinhole, filter, and gain settings are used.
>> Cold reboot does not fix the problem though no error messages are 
>> reported. The single fix so far is to let the system cool down for 
>> several hours then it is again working for few more hours.
>> Surprisingly all cooling fans are spinning normally as well as  room 
>> conditioning system.
>> 
>> 
>> 
>> 
>> We are off service contract so any feedback/advice what could be wrong
> 
>> and how to fix it is greatly appreciated.
>> 
>> 
>> Thank you,
>> Arvydas
>> 
>> 
>> 
>> 
>> 
>> Arvydas Matiukas, Ph.D.
>> Director of Confocal&Two-Photon Imaging Core Facility Department of 
>> Pharmacology SUNY Upstate Medical University
>> 766 Irving Ave., WH 3159
>> Syracuse, NY 13210
>> tel.: 315-464-7997
>> fax: 315-464-8014
>> email: [log in to unmask]