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Dear All,

I'm currently trialling 2 silicone oil immersion lenses from Olympus (30x/1.05NA and 60x/1.3NA) for live cell imaging on our Olympus FV1000 confocal, which is on an upright fixed stage microscope. So far, they both seem excellent and offer the higher level of resolution I'm looking for.

I am particularly interested in how these lenses compare with high NA water-immersion objective lenses. At the moment, we have long working distance water-immersion lenses or standard oil immersion so I don't have a high NA water-immersion objective lens to do my own comparison.

If anyone has personal experience with both types of lenses, I would be keen to hear their view.

I'm particularly interested in how the 60x/1.2NA water-immersion lens compares to the silicone oil immersion 60x/1.3NA, since I would like to buy one or the other.

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484

http://www.fmhs.auckland.ac.nz/sms/biru/