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You could try disconnecting the stage where it plugs in to the scanner. However this might introduce even more error messages as the stage will fail to initialise. Did you notice if the stage drive was hot (the plate that sticks out to the left of the stage). This can happen if you try to drive the stage when there is resistance ie if the sample was pushing against the objective. If you can start the software you could try changing the stage range of motion to the larger scale on the settings menu which might allow you to move it back to its central position. From your error message it sounds like the stage has moved outside its normal range. Did you try turning everything off and then restarting - that will reinitialise the hardware - I would try that first. Just rebooting the computer won't do this. It's the middle switch on the right hand side under the table.


Dr Lloyd Donaldson
Project Leader - Microscopy & Wood Identification
Manufacturing and Biomaterials
Scion - Forests, Products, Innovation
49 Sala Street, Rotorua 3010
Ph 07 343 5581
www.scionresearch.com




-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Shanna Banman
Sent: Friday, 29 June 2012 9:58 a.m.
To: [log in to unmask]
Subject: Leica SP5 Z-position Issue

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I just had a client in using the scope.  He had whole plant leaf mounts.  We did some Z-stacks, no issues - but when I went to change the objective to 10 for shut down the system and return the objective to the bottom position -4.00 mm, the value was +4.98 mm.  Has anyone run into this before?  Also, when I went to shut down the LAS AF program, there was an error about z-drive position was
35789867969 and must be between 0 and 254945956569 or something like that.
So i rebooted the system, and I cannot get into the LAS AF program because it keep giving me error and terminating. Can anyone help?



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