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Just out of curiosity, did you consider using a 50/50 beam splitter? That would mean no moving parts, and more stable alignment. I'd have thought that modern TiS lasers are powerful enough that both scopes would get enough. That way two users could do MP at once - at least if they could agree on a wavelength!
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
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Aust. Centre for Microscopy & Microanalysis, F09,
University of Sydney, NSW 2006
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cameron Nowell
Sent: Thursday, 7 June 2012 8:32 AM
To: [log in to unmask]
Subject: Re: two confocal systems on a single multiphoton laser?
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Hi Thomas,
We have exactly what you are talking about. We have a single MaiTai
DeepSee hooked up to two Olympus FV1000 systems (one inverted and one
upright). The IR induction is pretty simple to set up. Both IR induction
paths for the microscopes are standard (the inverted one was custom
built based on Olympus designs for political reasons - the list doesn't
need to know about this). Infornt of the output of the MaiTai is a
mirror on a precision motorized stage. When the stage is homed, the
mirror is in place to bounce the laser into one light path (in our case
the inverted) and when it is moved to the extreme of its travel the
laser goes straight into the induction path for the upright scope.
All of this takes up a fair bit of space obviously but ti does allow us
to have two multiphoton platforms that can also be used for single
photon imaging as well. We have even had people doing multi on one
platform and single on the other at the same time.
I am pretty sure that all the big four now offer this type of
configuration as an option when purchasing it.
Cheers
Cam
Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research Melbourne - Parkville Branch
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3158
Mobile: +61 422882700
Fax: +61 3 9341 3104
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Phillips, Thomas E.
Sent: Thursday, 7 June 2012 7:16 AM
To: [log in to unmask]
Subject: two confocal systems on a single multiphoton laser?
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Am I correct in assuming that there is no simple practical way to share
a single multiphoton laser with two confocal systems (e.g., one system
on an inverted frame for routine work and the other on an upright frame
dedicated for electrophysiology work)? If someone has actually
accomplished this, especially in a multi-user environment, I would
appreciate hearing about it. Thanks, Tom
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