CONFOCALMICROSCOPY Archives

June 2012

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From:
Matthew Nicholas <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 26 Jun 2012 13:30:04 -0400
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On Tue, Jun 26, 2012 at 11:41 AM, Gregg Jarvis <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Chroma dichroics should be hard oxide or sputtered, should be no
> problem for solvents.
>

Just to reiterate my earlier point regarding dichroic mirrors and
hard-drying glue (e.g. cyanoacrylate (Krazy glue) or nail polish) --
this was an issue I encountered when using Chroma mirrors (even on the
thicker 1.5mm versions specifically for lasers; I expect this would
happen with any mirror less than about 0.5" thick, and maybe even with
these). If you are using the dichroic in a pathway for a laser, it
will almost certainly mess up your beam profile when using these
glues/cements because they literally bends the mirror as they dry.
Conversely, a dab of soft-drying glue should be possible to apply in
such a way that there is no contact with the optical surface (only the
mirror edge -- if it is rather gelatinous/thick to start out with, it
will not be drawn onto the optical surface.). This can usually be
removed without solvent while leaving negligible residue. The other
issue with using solvents, even if the mirror can tolerate them, is
that you increase the risk of spreading the glue around unless you are
very careful.

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