*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello Hao, 
PerkinElmer have a team of technical support specialists that can help you with this, or any other imaging issues that you have. 

There are several ways to contact them, please follow the link below for details:

http://cellularimaging.perkinelmer.com/support/contact/

It's a good opportunity to mention that the team just don't just deal with conventional problems such a systems failures, but are very happy to advise with day to day imaging or analysis questions that you may have. 

Regards,
Andrew


Andrew Barlow PhD | Applications Specialist
PerkinElmer | For the Better	
[log in to unmask]
Phone:  + 44 2476 692229|   Fax:  +44 2476 690091   |   Mobile:   +44 7799 795 999
Millburn Hill Road, Coventry, CV4 7HS, UK
www.perkinelmer.com
Please consider the environment before printing this e-mail.
This e-mail message and any attachments are confidential and proprietary to PerkinElmer, Inc.  If you are not the intended recipient of this message, please inform the sender by replying to this email or sending a message to the sender and destroy the message and any attachments.  Thank you.

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Wu, Hao
> Sent: 12 July 2012 17:44
> To: [log in to unmask]
> Subject: how to use volocity to measure total fluorescence intensity?
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear All,
> I am wondering how to measure total fluorescence intensity using
> volocity. What I want to do is to circle out different region of a
> tissue or different area of a cell, and measure the total fluorescent
> intensity and compare these numbers. However, when I look at velocity
> tutorial, i could not find such a function.
> Can someone please give some advice?
> thank you so much
> Sincerely
> hao