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Hi Prabhakar:
We have successfully used DiI and DiO for staining blood derived macrophages (see:Blood 2009 114: 4664-4674). They are very photostable and used at the proper concentration relatively non-toxic.
Best wishes,
Chris
Christopher Guérin, Ph.D.
Leader, DMBR Microscopy Core
Manager, VIB Center for Life Science Imaging
V.I.B., D.M.B.R., Univ. Gent
Fiers-Schell-Van Montagu' building
Technologiepark 927, B-9052 Ghent (Zwijnaarde) - Belgium
tel : +32-9-33 13 611
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web:http://www.dmbr.ugent.be/ext/public/microscopy/index.html