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The Asi solution is indeed the only one that will work for old stands. It uses a combo of the focussing gadget and a piezo z controller which gives you more capabilities wrt to fast z control so this complements a spinning disk for example. It works well but is a big investment to throw at an old stand

Simon Watkins Ph.D
Professor and Vice Chair Cell Biology
Professor Immunology
Director Center for Biologic Imaging
University of Pittsburgh 
Bsts 225 3550 terrace st
Pittsburgh PA 15261
Www.cbi.pitt.edu
412-352-2277

On Jul 19, 2012, at 8:29, "Valeria Berno" <[log in to unmask]> wrote:

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> 
> Dear all
> 
> I would like to test an autofocus system on an old inverted widefield 
> microscope from Zeiss. The system is already motorized for stage, Z and 
> filters but the ability to keep focus during time lapse or in different 
> well plates is really challenging...
> 
> Have anyone tried the CRISP system from ASI? This seems to be the only 
> system that can be integrated in old stands, but I have been asked for 
> money for the demo and I would like to hear some feedback from users 
> before investing money....
> 
> thanks in advance
> 
> Valeria
> 
> -- 
> Valeria Berno,PhD
> Microscopy Facility Manager
> MRC Centre for Regenerative Medicine
> SCRM building
> The University of Edinburgh
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> 
> 
> 
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