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Hello,

We have DAPI stained nuclei in cultured mammalian cells and they are
typically not homogeneously fluorescent, with obvious areas of very low
intensity, such as the nucleolus, but also a patchy-ness in the rest of the
nucleus with varying intensity throughout. For us this is most easily seen
in confocal or deconvolved-wf slices. Does anyone know, or have any
theories on, what these 'darker' areas are a result of?

Some thoughts we had are regions relatively deficient in DNA, or
heterochomatin rich regions? Or is it just a result of differential binding
as a result of A-T rich regions?

Any thoughts on this will be much appreciated.

Thanks,
Graham

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*Dr Graham Wright*
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

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