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By definition, Lamin is not in the nuclear membrane but underneath. So
depending on what your question and your planed resolution is, this may
or may not be a problem.
Have a look at this comparison of anti-nuclear pore and anti-lamin with
confocal and 3D-SIM by Schermelleh et al.
https://commons.wikimedia.org/wiki/File:3D-SIM-1_NPC_Confocal_vs_3D-SIM.jpg
(make sure you don't loose the part of the URL in the second line, link
to the publication is in the figure legend)
Note that anti-NPC is rather homogeneous in confocal but not in 3D-SIM.
Good luck
Steffen
On 12.09.2012 12:56, Cameron Nowell wrote:
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>
> Howdy,
>
> I would second the suggestion of Lamin. We have had some spectacular images generated using it.
>
> Cheers
>
> Cam
>
>
> Cameron J. Nowell
> Microscpy Manager
> Centre for Advanced Microscopy
> Ludwig Insttue for Cancer Research
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>
> ________________________________
>
> From: Confocal Microscopy List on behalf of Jean-Marie Vanderwinden
> Sent: Wed 12/09/2012 6:49 PM
> To: [log in to unmask]
> Subject: Re: nuclear membrane marker
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> Dear,
>
> You may consider LaminA/C:
>
> fluorescent constructs for live imaging
> e.g. http://www.ncbi.nlm.nih.gov/pubmed?term=20079404
> and Lamin Abs work usually fine on fixed material...
>
> With best wishes.
>
> JM
>
> At 09:35 12/09/2012, you wrote:
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>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Vitaly,
>>
>> Frankly, there is no really specific nuclear membrane dye. I remember a
>> paper from Zal et al., (Traffic 2006; 7: 16071613), which says the dye
>> FM4-64 is specific for the nuclear membrane, though in our tests the results
>> were inconsistent and it also stained other membrane entities.
>> I would suggest to use anti-NPC antibodies - these should specifically stain
>> the nuclear membrane (in interphase cells) - famous in the field is mAb414.
>>
>> hope this helps,
>> Josef
>>
>> On Tue, 11 Sep 2012 14:57:00 -0700, Vitaly Boyko
>> <[log in to unmask]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
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>>>
>>> Dear All,
>>>
>>> one of our users is looking for a relatively abundant marker that is
>> homogeneously distributed over the nuclear membrane in cultured human cells
>> (it could be a well characterized Ab against the nuclear receptor, or simply
>> specific (nuclear) membrane stain).
>>>
>>> Many thanks in advance,
>>>
>>> Vitaly
>
> Jean-Marie.
>
> Jean-Marie Vanderwinden, M.D., Ph.D.
> Directeur de Recherche F.N.R.S. / Research
> Director, National Fund for Scientific Research (Belgium)
> Neurophysiology lab http://www.ulb.ac.be/medecine/neurophy/
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> Postal address:
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> (: Secretary: +(32).2.555.42.30, Direct: +(32).2.555.69.88
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--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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