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Further to David's email - we also use something similar, a 2-part dental
silicone. The product is from PicoDent and called TwinSil (
http://www.picodent.de/artikel/831-picodent_twinsilsupsup_22.html?anz=0)
and our local Leica people were able to help us get hold of some after we
saw them using it for sample prep on the GSDIM system (no commercial
interest in either).

After you mix the two solutions you have a few minutes to use it before it
sets and seals. The nice thing is that it peals off quite easily afterwards
if you have to retrieve the coverslip for something else.

Regards,
Graham

-- 
Dr Graham Wright
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

E:   [log in to unmask]
W:  http://www.imb.a-star.edu.sg/imu/

On 19 September 2012 05:35, David Baddeley <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Something we've used on occasion is 2-part dental silicone (the same stuff
> that is used for making impression moulds) - the exact name and supplier
> will vary depending on where you are. You mix the parts (I tend to use a
> small weighing dish to mix in), and apply a bead round the edge of the
> coverslip (I usually use a pippette tip for this). I've mostly used it for
> fixed cells, but have heard it touted as being good for live cells (if you
> can put it in your mouth, it can't be particularly toxic) . It also sets
> slightly faster than nail-polish, which is helpful if you are under time
> pressure.
>
> cheers,
> David
>
>
> ________________________________
>  From: Chris Booth <[log in to unmask]>
> To: [log in to unmask]
> Sent: Wednesday, 19 September 2012 8:58 AM
> Subject: Re: Sealing cover slips
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> This is a non-commercial post. In our lab we use an organic coverslip
> sealer
> from the company Biotium called CoverGrip. It comes in the same kind of
> container as nail polish, with a little brush attached to the lid for ease
> of use.
> I've had great luck using this product and the price is extremely fair for
> a RTU
> reagent, especially if you don't have the ingredients to make a custom
> cocktail.
>
> Definition from the site: CoverGrip Coverslip Sealant is made with
> d-limonene, a
> natural, environmentally-friendly solvent with a pleasant citrus aroma,
> and dries
> to form a clear, hard, durable seal along the edges of the coverslip.
>
> http://biotium.com/product/product_types/search/price_and_info.asp?
> item=23005
>