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December 2012

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Subject:
From:
Aleem Syed <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 14 Dec 2012 12:05:45 -0600
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

And also do not use the computer for anything during scanning. It can give
you artifacts in the final image.

~aleem

On Fri, Dec 14, 2012 at 12:03 PM, Aleem Syed <[log in to unmask]> wrote:

> Increase your exposure time per pixel. It may help, did help in my case.
>
> Good luck.
>
> ~Aleem
>
>
> On Fri, Dec 14, 2012 at 11:59 AM, Craig Brideau <[log in to unmask]>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> It is also possible that the electronics are not grounded properly.  You
>> can get electronic noise from an improper ground loop.  It's also possible
>> you have light leaking in from somewhere; I've gotten stripes in images
>> from flashing LEDs on equipment near the microscope.  A quick piece of
>> black tape over the offending LED and no more noise!
>>
>> Craig
>>
>>
>> On Thu, Dec 13, 2012 at 11:46 PM, Pascal Weber <[log in to unmask]
>> >wrote:
>>
>> > *****
>> > To join, leave or search the confocal microscopy listserv, go to:
>> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> > *****
>> >
>> > Hi everebody,
>> >
>> > I had heard about the problem of sensitivity of PMT has been set by a
>> > technician
>> > linearization sensitivity. (Procedure to apply)
>> > For sensitivity to mobile I am very surprised and I do the same test!
>> >
>>
>
>
>
> --
>
>


-- 
Aleem Syed
Graduate Student,
Dr. Smith Group,
0712 Gilman Hall,
Iowa State University
Ames 50011
USA

*Success and Failure are part of life, truth is none of 'em  is permanent,
including Life....*

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