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Hi Caroline,
Yes, you can do this. I have published an article where I used that
algorithm in ImageJ.
Here is my reference:
Van Breda, S. V., van der Merwe, C. F., Robbertse, H., & Apostolides, Z.
(2012). Immunohistochemical localization of caffeine in young Camellia
sinensis (L.) O. Kuntze (tea) leaves. Planta, 1-10.
The reference of the algorithm is:
Forster B, D van de Ville, J Berent, D Sage, M Unser (2004) Complex wavelets
for extended
depth-of-field: a new method for the fusion of multichannel microscopy
images. Microsc Res
Techniq 65:33-42
Or search for EDF (extended depth of field). Hope this helps.
Regards,
Shane
----- Original Message -----
From: "bluec" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, January 18, 2013 5:00 PM
Subject: Z stacks merging the image slices into one
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Hello All
I would greatly appreciate if someone could suggest a method to merge the
slices from a z-stack into just one image? Is it possible to do this with
image J?
Thank you
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