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February 2013

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Confocal Microscopy List <[log in to unmask]>
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Thu, 28 Feb 2013 12:13:38 +0000
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*****
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I think we need a bit more explanation before this becomes even remotely comprehensible!  But your hard work is certainly appreciated.

                                                                     Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of George McNamara
Sent: Thursday, 28 February 2013 11:03 PM
To: [log in to unmask]
Subject: Re: Video compression

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I posted to    http://works.bepress.com/gmcnamara/27/     an Excel file 
that can be downloaded. The file contains my evaluation of a large number of codec's to make AVI files from a monochrome data set. 
Evaluation was done with MetaMorph in 2005-2006. Summary (first worksheet tab of the Excel file):

filename                                                            
filesize    Score (sum)
VP61 Advanced Profile                              1,582,592    1
VP62 Heightened Sharpness                       1,584,640    1
VP60 Simple Profile                                   1,612,288    1
DivX MPEG-4 Fast-Motion                       2,459,648    5
DivX MPEG-4 Low-Motion                      4,312,064    4
Microsoft MPEG-4 V2                              6,698,496    2
Intel Indeo video R3.2                              20,154,880    6
Microsoft Windows Media Video 9          28,512,768    2
Cinepak                                                   35,144,192    2
Intel Indeo Video 4.5                               38,092,800    2
Microsoft Video 1                                   65,801,728    3
QuickTime                                            225,830,571    4
uncompressed                                       363,842,048    2
average score (of 24 codecs)                  50,512,800    3.16
standard deviation                                   88,101,414    2.13
median                                                   13,934,080    2
maximum possible score (high is bad)                           12

Enjoy,

George
p.s. I am presenting my latest Tattletales poster on how to multiplex fluorescent reporters in live cells, poster at ABRF and CSHL (single cells analyses workshop) next week. I hope some of you can attend. The current poster and two page abstract are the most recent two PDF files inside the zip downloadable from http://works.bepress.com/gmcnamara/26/
I expect to have data by end of summer (2013).

On 2/27/2013 7:55 AM, Guy Cox wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Jasc Software Paint Shop Pro also comes with Animation Shop which offers a wide range of compression options.  Some are terrible, others are good - it needs a fair bit of experimentation.  But it is low cost software.  I use it a lot, but otherwise have no commercial connection.
>
>                                     Guy
>
> -----Original Message-----
> From: Confocal Microscopy List 
> [mailto:[log in to unmask]] On Behalf Of Simon Walker
> Sent: Wednesday, 27 February 2013 11:06 PM
> To: [log in to unmask]
> Subject: Video compression
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List,
> Can anyone recommend software for video compression (either free or 
> licensed)?  We have some large files from live cell imaging exps that 
> we need to submit to a journal and somehow get them down to less than 
> 10 MB (this size limit seems crazy in this day and age, but maybe 
> there are still people using dial-up to access journals..).  What 
> codecs/file formats are people using to obtain the best compression with minimal loss of information?
> Thanks,
> Simon
>
>    

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