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Ibidi makes many versions of chambers. Some do have glass bottoms. We routinely use two designs in our lab with #1.5 coverslips on the bottom, but we want the larger open chambers for other experiments.
Regards,
Michael
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Myriam Gastard
Sent: Wednesday, March 13, 2013 2:10 PM
To: [log in to unmask]
Subject: Re: Nunc Lab-Tek problem
Hi Michael,
Be aware that Ibidi slides bottom are not made of glass and will interfere with a lot of reflection during imaging.
Hope this will help,
Myriam
Myriam Gastard, PhD
From my iPad.
On Mar 13, 2013, at 12:08 PM, "Deepak Saini" <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Michael,
>
> Look at ibidi they have quite a few designs and may be some can fit
> your requirements.
>
> No commercial interest.
>
> Deepak
>
>
> On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael
> <[log in to unmask]
>> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi.
>>
>> For many years we used Nunc Lab-Tek chambered coverglasses. They were
>> great.
>>
>> However, they recently began shipping a different design with the
>> same product number. The chambers no longer fit in our microscope
>> stages inserts. Also, the volume changed.
>> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/
>>
>> While we can adjust to the volume change, the plastic tab at the end
>> of the chamber is a big problem.
>>
>> I thought of having people cut the tab off before doing experiments,
>> but this would produce a lot of plastic dust (and we'd have to buy a
>> suitable saw or dremel).
>>
>> Does anybody know of an alternative manufacturer or if the old style
>> chambers are still available from somewhere?
>>
>> Thanks!
>>
>> Michael
>>
>>
>>
>>
>> ________________________________________________________
>> Michael Cammer, Assistant Research Scientist Skirball Institute of
>> Biomolecular Medicine
>> Lab: (212) 263-3208 Cell: (914) 309-3270
>>
>
>
>
> --
> ----------------------------------------------------------------------
> ------------------------------
> Deepak K Saini, PhD
> Assistant Professor
> Department of Molecular Reproduction, Development and Genetics Indian
> Institute of Science (IISc) Bangalore, India.
> [log in to unmask]
> [log in to unmask]
> Tel: 080-22932574
> ----------------------------------------------------------------------
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> <[log in to unmask]>
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