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Prabhakar:

Just one more comment:

As was pointed out by Gabriel, the Chamlide incubator does not monitor CO2 inside the chamber, as far as I know (no evidence of any sensor in there). It probably just mixes CO2 and Air and delivers that to the chamber. The chamber is small and engineered pretty tight though, and we use very little CO2 (2-3 sbps, or "small bubbles per second"). I don't know if the system from Pathology Devices monitors CO2 or not.

On the other hand, In Vivo scientific does have an incubator system where CO2 is monitored right inside the incubation chamber:

http://www.invivoscientific.com/Peripherals.htm

I believe this system though is designed to work with the larger type of incubator (that wraps around the scope) for temperature control. There is a second chamber that fits inside and holds the specimen. The CO2 probe goes in there. I think the key is to have a chamber that is well sealed, otherwise there is too much exchange of gas with the outside and CO2 control will be erratic.  You may want to check the exact specs with the vendor if interested. 

--
Julio Vazquez,
Fred Hutchinson Cancer Research Center
 




On Mar 13, 2013, at 10:32 AM, B. Prabhakar Pandian wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Hello All,
>                  We are looking to buy a stage top incubator. However, one of the questions which has come up is whether the gases are running continuously to maintain levels since it is open near the objective.
> Are there gaskets to seal around slides/petri dishes so that a feedback on the levels can be provided and the gas flow can be upregulated or downregulated depending on the need.   Finally, most of them
> have a bottle of water for humidifying the chamber. Only Tokai Hit has tray with water in the insert itself. Any thoughts on the pros/cons is appreciated.
> 
> Any other feedback to help us make a sound decision will be great.
> 
> Thanks,
> 
> -Prabhakar