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Sure, the Bio-Rad merged image put each channel into 4 bits. But the side - by- side image contained each channel at full 8 bit resolution, 384 * 512, in greyscale. I keep an old (but powerful) computer running XP just so that I can use the old utilities such as Confocal Assistant. But with any imaging program you can cut the picture in two - just make sure you get it exact - and then combine the two 384 * 512 greyscale images into an RGB image.
As to the 4-bit merged images, I did write a program to uncombine them. Whether it will run under XP I'm not sure (it is a DOS program). Of course you still only have 4 bits of data, but once they are each in an 8-bit image you can at least open them! If you have precious data saved in this format, send me a sample image and I'll try, but please not until after Easter since I'm away at the moment (snowed up in England on the way to FOM in Maastricht).
Guy
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cammer, Michael
Sent: Wednesday, 13 March 2013 1:50 AM
To: [log in to unmask]
Subject: Re: side-by-side merge
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But the original BioRad merge feature made each channel only 4 bits and scrambled the LUT in an odd way, so don't rue too much...
If anyone has an importer, converter etc to open these old .pic merged images, please let me know!
Regards,
Michael C.
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Unruh, Jay
Sent: Tuesday, March 12, 2013 10:41 AM
To: [log in to unmask]
Subject: Re: side-by-side merge
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There are a couple of ways to do this in ImageJ. First you need to split the colors with Image>Color>Split Channels. If you want to merge them side by side permanently, convert to RGB (Image>Type>RGB Color) use Image>Stacks>Tools>Combine. If you just want to browse them together, use Analyze>Tools>Sync Windows.
Jay
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Michal Opas
Sent: Tuesday, March 12, 2013 8:35 AM
To: [log in to unmask]
Subject: side-by-side merge
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Dear Listers,
This harks back to prhistory:
on Bio-Rad MRC 600 and Confocal Assistant by Todd Brelje one could merge dual wavelength images taken simultaneously using split screen ("split
box") feature of the scope.
This "side-by-side merge" feature was very convenient for the dual excitation, split image frames as one could quickly merge left with right and see how good the merged image is.
Sadly, the Confocal Assistant will not work under 64-bit OS.
I look for a functionality allowing for "side-by-side merge" in ImageJ and Fiji but failed to find it.
I am discombobulated: is such a useful feature gone forever (past Win
XP) or my ineptitude is gross?
Thank you very much for advice.
Michal
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